Anti-AAG/MPG Antibody Picoband™

Product Name

Anti-AAG/MPG Antibody Picoband™

View all MPG Antibodies

SKU/Catalog Number

A00037

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AAG/MPG Antibody Picoband™ catalog # A00037. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-AAG/MPG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00037)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human AAG/MPG recombinant protein (Position: Q20-A298).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00037 is reactive to MPG in Human

Applications

A00037 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

32.869kDa

Background of MPG

MPG(N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.TheMPGgene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized humanMPGgene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine(meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human
Direct ELISA, 0.1-0.5 μg/ml/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of AAG/MPG using anti-AAG/MPG antibody (A00037).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAG/MPG antigen affinity purified polyclonal antibody (Catalog # A00037) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAG/MPG at approximately 37 kDa. The expected band size for AAG/MPG is at 33 kDa.

Gene/Protein Information For MPG (Source: Uniprot.org, NCBI)

Alternative Names

3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; AAG; ADPG; anpg; APNG; CRA36.1 (3-methyl-adenine DNA glycosylase); CRA36.1; DNA-3-methyladenine glycosylase; EC 3.2.2.21; MDGalkyladenine DNA glycosylase; Mid1; N-methylpurine-DNA glycosylase, MPG; N-methylpurine-DNA glycosylasePIG16; PIG11; proliferation-inducing protein 11; proliferation-inducing protein 16 MPG AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg N-methylpurine DNA glycosylase DNA-3-methyladenine glycosylase|3 end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p|3-alkyladenine DNA glycosylase|3-methyladenine DNA glycosidase|CRA36.1 (3-methyl-adenine DNA glycosylase)|N-methylpurine-DNA glycosylase, MPG|proliferation-inducing protein 11|proliferation-inducing protein 16

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In this infographic, you will see the following information for MPG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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